CONFERENCE PROCEEDING
Effect of smoking on salivary free amino acid levels
 
More details
Hide details
1
Department of Periodontology, Faculty of Dentistry, Ankara University, Ankara, Turkey
2
Department of Medical Biochemistry, School of Medicine, Acıbadem University, Ankara, Turkey
Publish date: 2018-10-03
 
Tob. Induc. Dis. 2018;16(Suppl 3):A34
KEYWORDS:
Download abstract book (PDF)

ABSTRACT:
Objective:
The aim of this study was to identify probable intermediate biomarkers of disturbed pathways and their link between smoking.

Methods:
Un-stimulated whole saliva and serum samples were collected from a total of 30 systemically healthy participants with periodontally healthy smokers (S) (n=15) and non-smokers (n=15). Periodontal indices (plaque index, gingival index, probing depth, bleeding on probing, clinical attachment level) were recorded to confirm periodontal health. Saliva was purified, and a total of 28 amino acids and metabolites were analyzed by liquid chromatography-mass spectrometry (LC-MS/MS). Smoking status was validated measuring serum cotinine levels. Intergroup comparisons were assessed using the Mann Whitney U test.

Results:
When 28 amino acids were evaluated, smokers had statistically significantly higher cystathionine levels than non-smokers (p<0.05).

Conclusions:
Saliva cystathionine is associated with smoking in periodontally healthy individuals, and is possibly related to altered sulfuration pathway.

CORRESPONDING AUTHOR:
Cavid Mammadov   
Department of Periodontology, Faculty of Dentistry, Ankara University, Ankara, Turkey
eISSN:1617-9625