Lethal impacts of cigarette smoke in cultured tobacco cells
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Laboratory of Chemical Biology and Bioengineering, Faculty and Graduate School of Environmental Engineering, The University of Kitakyushu, Kitakyushu 808-0135, Japan
Submission date: 2011-01-14
Acceptance date: 2011-07-16
Publication date: 2011-07-16
Corresponding author
Tomonori Kawano   

Laboratory of Chemical Biology and Bioengineering, Faculty and Graduate School of Environmental Engineering, The University of Kitakyushu, Kitakyushu 808-0135, Japan
Tobacco Induced Diseases 2011;9(July):8
In order to understand and generalize the toxic mechanism of cigarette smoke in living cells, comparison of the data between animal systems and other biological system such as microbial and plant systems is highly beneficial.

By employing the tobacco cells as model materials for cigarette smoke toxicity assay, the impacts of the combustion by-products such as nitrogen oxides could be highlighted as the toxic impacts of the plantderived endogenous chemicals could be excluded in the plant cells.

Cigarette smoke-induced cell death was assessed in tobacco cell suspension cultures in the presence and absence of pharmacological inhibitors.

Cigarette smoke was effective in induction of cell death. The smoke-induced cell death could be partially prevented by addition of nitric oxide (NO) scavenger, suggesting the role for NO as the cell death mediator. Addition of NO donor to tobacco cells also resulted in development of partial cell death further confirming the role of NO as cell death mediator. Members of reactive oxygen species and calcium ion were shown to be protecting the cells from the toxic action of smoke-derived NO.

Merz PG, Keller-Stanislawski B, Huber T, Woodcock BG, Rietbrock N: Transdermal nicotine in smoking cessation and involvement of nonspecific influences. Int J Clin Pharmacol Ther Toxicol. 1993, 31: 476-82.
Mozaffarieh M, Konieczka K, Hauenstein D, Schoetzau A, Flammer J: Half a pack of cigarettes a day more than doubles DNA breaks in circulating leukocytes. Tobacco Induced Diseases. 2010, 8: 14-10.1186/1617-9625-8-14.
Ojima M, Hanioka T: Destructive effects of smoking on molecular and genetic factors of periodontal disease. Tobacco Induced Diseases. 2010, 8: 4-10.1186/1617-9625-8-4.
Walker A, Udupa KB, Chowdhury P: Mitogenic and functional responses by nicotine and hydrogen peroxide in AR42J cells: a comparative study. Tobacco Induced Diseases. 2008, 4: 5-10.1186/1617-9625-4-5.
Lewis RS, Bowen SW, Keogh MR, Dewey RE: Three nicotine demethylase genes mediate nornicotine biosynthesis in Nicotiana tabacum L.: Functional characterization of the CYP82E10 gene. Phytochemistry. 2010, 71: 1988-98. 10.1016/j.phytochem.2010.09.011.
Kawano T, Furuichi T, Muto S: Controlled free salicylic acid levels and corresponding signaling mechanisms in plants. Plant Biotechnol. 2004, 21: 319-335. 10.5511/plantbiotechnology.21.319.
Jones AM, Chory J, Dangl JL, Estelle M, Jacobsen SE, Meyerowitz EM, Nordborg M, Weigel D: The impact of Arabidopsis on human health: Diversifying our portfolio. Cell. 2008, 13: 939-943.
Muller T, Haussmann HJ, Schepers G: Evidence for peroxynitrite as an oxidative stress-inducing compound of aqueous cigarette smoke fractions. Carcinogenesis. 1997, 18: 295-301. 10.1093/carcin/18.2.295.
Kadono T, Yamaguchi Y, Furuichi T, Hirono M, Garrec J-P, Kawano T: Ozone-induced cell death mediated with oxidative and calcium signaling pathways in tobacco Bel-W3 and Bel-B cell suspension cultures. Plant Signal Behav. 2006, 1: 312-22. 10.4161/psb.1.6.3518.
Kadono T, Tran D, Errakhi R, Hiramatsu T, Meimoun P, Briand J, Iwaya-Inoue M, Kawano T, Bouteau F: Increased anion channel activity is an unavoidable event in ozone-induced programmed cell death. PLoS ONE. 2010, 5 (10): e13373-10.1371/journal.pone.0013373.
Lin C, Yu Y, Kadono T, Iwata M, Umemura K, Furuichi T, Kuse M, Isobe M, Yamamoto Y, Matsumoto H, Yoshizuka K, Kawano T: Action of aluminum, novel TPC1-type channel inhibitor, against salicylate-induced and cold shock-induced calcium influx in tobacco BY-2 cells. Biochem Biophys Res Commun. 2005, 332: 823-30. 10.1016/j.bbrc.2005.05.030.
Kagenishi T, Yokawa K, Kuse M, Isobe M, Bouteau F, Kawano T: Prevention of copper-induced calcium influx and cell death by prion-derived peptide in suspension-cultured tobacco cells. Z Naturforsch. 2009, 64c: 441-17.
Kawano T, Sahashi N, Takahashi K, Uozumi N, Muto S: Salicylic acid induces extracellular generation of superoxide followed by an increase in cytosolic calcium ion in tobacco suspension culture: The earliest events in salicylic acid signal transduction. Plant Cell Physiol. 1998, 39: 721-30.
Zhang J, Juedes N, Narayan VM, Yue B, Rockwood AL, Palma NL, Patel JM: A cellular model to mimic exhaled cigarette smoke-induced lung microvascular endothelial cell injury and death. Int J Clin Exp Med. 2010, 3: 223-232.
Hoffmann D, Rivenson A, Hecht SS: The biological significance of tobacco-specific N-nitrosamines: Smoking and adenocarcinoma of the lung. Crit Rev Toxicol. 1996, 26: 199-211. 10.3109/10408449609017931.
Wynder EL, Muscat JE: The changing epidemiology of smoking and lung cancer histology. Environ Health Perspect. 1995, 103: 143-148.
Hoffmann D, Hoffmann I, El-Bayoumy K: The less harmful cigarette: A controversial issue. A tribute to Ernst L. Wynder. Chem Res Toxicol. 2001, 14: 767-790. 10.1021/tx000260u.
Calogero A, Polosa R, Perdichizzi A, Guarino F, La Vignera S, Scarfia A, Fratantonio E, Condorelli R, Bonanno O, Barone N, Burrello N, D'Agata R, Vicari E: Cigarette smoke extract immobilizes human spermatozoa and induces sperm apoptosis. Reprod BioMed Online. 2009, 19: 564-571. 10.1016/j.rbmo.2009.05.004.
Zhu X, Caplan J, Mamillapalli P, Czymmek K, Dinesh-Kumar SP: Function of endoplasmic reticulum calcium ATPase in innate immunity-mediated programmed cell death. EMBO J. 2010, 29: 1007-1018. 10.1038/emboj.2009.402.
Boursiac Y, Lee SM, Romanowsky SM, Blank RR, Sladek C, Chung WS, Harper JF: Disruption of the vacuolar calcium-ATPases in Arabidopsis results in the activation of a salicylic acid-dependent programmed cell death pathway. Plant Physiol. 2010, 154: 1158-1171. 10.1104/pp.110.159038.
De Michele R, Vurro E, Rigo C, Costa A, Elviri L, Di Valentin M, Careri M, Zottini M, di Toppi LS, Lo Schiavo F: Nitric oxide is involved in cadmium-induced programmed cell death in Arabidopsis suspension cultures. Plant Physiol. 2009, 150: 217-228. 10.1104/pp.108.133397.
Beligni MV, Fath A, Bethke PC, Lamattina L, Jones RL: Nitric oxide acts as an antioxidant and delays programmed cell death in barley aleurone layers. Plant Physiol. 2002, 129: 1642-1650. 10.1104/pp.002337.
Ahlfors R, Brosché M, Kollist H, Kangasjärvi J: Nitric oxide modulates ozone-induced cell death, hormone biosynthesis and gene expression. Arabidopsis thaliana Plant J. 2009, 58: 1-12.
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