RESEARCH PAPER
Effects of whole cigarette smoke on human beta defensins expression and secretion by oral mucosal epithelial cells
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Pei Ye 1
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1
Department of Oral Medicine, Institute and Hospital of Stomatology, Nanjing University Medical School, Nanjing, China
 
2
Department of Prosthodontics, Institute and Hospital of Stomatology, Nanjing University Medical School, Nanjing, China
 
3
Immunology and Reproduction Biology Laboratory, Medical School, Nanjing University, Nanjing, China
 
 
Submission date: 2014-08-26
 
 
Acceptance date: 2015-01-10
 
 
Publication date: 2015-01-24
 
 
Corresponding author
Wen-mei Wang   

Department of Oral Medicine, Institute and Hospital of Stomatology, Nanjing University Medical School, 30 Zhongyang Road, Nanjing 210008, China
 
 
Xiang Wang   

Immunology and Reproduction Biology Laboratory, Medical School, Nanjing University, 22 Hankou Road, Nanjing 210093, China
 
 
Tobacco Induced Diseases 2015;13(January):3
 
KEYWORDS
ABSTRACT
Background:
Cigarette smoke a recognized risk factor for many systemic diseases and also oral diseases. Human beta defensins (HBDs), a group of important antimicrobial peptides expressed by the epithelium, are crucial for local defense and tissue homeostasis of oral cavity. The aim of this study was to evaluate potential effects of whole cigarette smoke (WCS) exposure on the expression and secretion of HBDs by oral mucosal epithelial cells.

Methods:
Immortalized human oral mucosal epithelial (Leuk-1) cells were exposed to WCS for various time periods. HBD-1, -2 and -3 expression and subcellular localization were detected by real time qPCR, immunofluorescence assay and confocal microscopy. According to the relative fluorescent intensity, the expression levels of HBD-1, -2 and -3 were evaluated by digital image analysis system. The alteration of HBD-1, -2 and -3 secretion levels was measured by the Enzyme-Linked Immunosorbent Assay.

Results:
WCS exposure remarkably attenuated HBD-1 expression and secretion while clearly enhanced HBD-2, -3 expression levels and HBD-2 secretion by Leuk-l cells. It appeared that there was no significant effect of WCS exposure on HBD-3 secretion.

Conclusions:
WCS exposure could modulate expression and secretion of HBDs by oral mucosal epithelial cells, establishing a link between cigarette smoke and abnormal levels of antimicrobial peptides. The present results may give a new perspective to investigate smoking-related local defense suppression and oral disease occurrence.

 
REFERENCES (38)
1.
Chung WO, Dommisch H, Yin L, Dale BA. Expression of defensins in gingiva and their role in periodontal health and disease. Curr Pharm Des. 2007;13(30):3073–83.
 
2.
Wang X, Jiang W, Duan N, Qian Y, Zhou Q, Ye P, et al. NOD1, RIP2 and Caspase12 are potentially novel biomarkers for oral squamous cell carcinoma development and progression. Int J Clin Exp Pathol. 2014;7(4):1677–86.
 
3.
Diamond G, Ryan L. Beta-defensins: what are they really doing in the oral cavity? Oral Dis. 2011;17(7):628–35.
 
4.
Ganz T. The role of antimicrobial peptides in innate immunity. Integr Comp Biol. 2003;43(2):300–4.
 
5.
Jarczak J, Kosciuczuk EM, Lisowski P, Strzalkowska N, Jozwik A, Horbanczuk J, et al. Defensins: natural component of human innate immunity. Hum Immunol. 2013;74(9):1069–79.
 
6.
Zhang L, Ren JW, Wong CC, Wu WK, Ren SX, Shen J, et al. Effects of cigarette smoke and its active components on ulcer formation and healing in the gastrointestinal mucosa. Curr Med Chem. 2012;19(1):63–9.
 
7.
Bunnell A, Pettit N, Reddout N, Sharma K, O'Malley S, Chino M, et al. Analysis of primary risk factors for oral cancer from select US states with increasing rates. Tob Induc Dis. 2010;8:5.
 
8.
Gorsky M, Epstein JB, Hasson-Kanfi H, Kaufman E. Smoking habits among patients diagnosed with oral lichen planus. Tob Induc Dis. 2004;2(2):103–8.
 
9.
Phillips J, Kluss B, Richter A, Massey E. Exposure of bronchial epithelial cells to whole cigarette smoke: assessment of cellular responses. Altern Lab Anim. 2005;33(3):239–48.
 
10.
Gualerzi A, Sciarabba M, Tartaglia G, Sforza C, Donetti E. Acute effects of cigarette smoke on three-dimensional cultures of normal human oral mucosa. Inhal Toxicol. 2012;24(6):382–9.
 
11.
Semlali A, Chakir J, Goulet JP, Chmielewski W, Rouabhia M. Whole cigarette smoke promotes human gingival epithelial cell apoptosis and inhibits cell repair processes. J Periodontal Res. 2011;46(5):533–41.
 
12.
Semlali A, Chakir J, Rouabhia M. Effects of whole cigarette smoke on human gingival fibroblast adhesion, growth, and migration. J Toxicol Environ Health A. 2011;74(13):848–62.
 
13.
Colombo G, Dalle-Donne I, Orioli M, Giustarini D, Rossi R, Clerici M, et al. Oxidative damage in human gingival fibroblasts exposed to cigarette smoke. Free Radic Biol Med. 2012;52(9):1584–96.
 
14.
Pierson T, Learmonth-Pierson S, Pinto D, van Hoek ML. Cigarette smoke extract induces differential expression levels of beta-defensin peptides in human alveolar epithelial cells. Tob Induc Dis. 2013;11(1):10.
 
15.
Semlali A, Witoled C, Alanazi M, Rouabhia M. Whole cigarette smoke increased the expression of TLRs, HBDs, and proinflammory cytokines by human gingival epithelial cells through different signaling pathways. PLoS One. 2012;7(12):e52614.
 
16.
Thorne D, Adamson J. A review of in vitro cigarette smoke exposure systems. Exp Toxicol Pathol. 2013;65(7–8):1183–93.
 
17.
Adamson J, Thorne D, McAughey J, Dillon D, Meredith C. Quantification of cigarette smoke particle deposition in vitro using a triplicate quartz crystal microbalance exposure chamber. Biomed Res Int. 2013;2013:685074.
 
18.
Adamson J, Azzopardi D, Errington G, Dickens C, McAughey J, Gaca MD. Assessment of an in vitro whole cigarette smoke exposure system: the Borgwaldt RM20S 8-syringe smoking machine. Chem Cent J. 2011;5:50.
 
19.
Thorne D, Wilson J, Kumaravel TS, Massey ED, McEwan M. Measurement of oxidative DNA damage induced by mainstream cigarette smoke in cultured NCI-H292 human pulmonary carcinoma cells. Mutat Res. 2009;673(1):3–8.
 
20.
Maunders H, Patwardhan S, Phillips J, Clack A, Richter A. Human bronchial epithelial cell transcriptome: gene expression changes following acute exposure to whole cigarette smoke in vitro. Am J Physiol Lung Cell Mol Physiol. 2007;292(5):L1248–56.
 
21.
Wolgin M, Liodakis S, Ulrich I, Zakrzewicz A, Kielbassa AM, Pries AR. Gene expression of human beta defensins-1 and -2 is significantly reduced in non-inflamed keratinized oral tissue of smokers. J Dent. 2012;40(11):949–54.
 
22.
Bogefors J, Kvarnhammar AM, Hockerfelt U, Cardell LO. Reduced tonsillar expression of human beta-defensin 1, 2 and 3 in allergic rhinitis. FEMS Immunol Med Microbiol. 2012;65(3):431–8.
 
23.
He P, Wang AG, Xia T, Gao P, Niu Q, Guo LJ, et al. Mechanism of the neurotoxic effect of PBDE-47 and interaction of PBDE-47 and PCB153 in enhancing toxicity in SH-SY5Y cells. Neurotoxicology. 2009;30(1):10–5.
 
24.
Bazzini C, Rossetti V, Civello DA, Sassone F, Vezzoli V, Persani L, et al. Short- and long- term effects of cigarette smoke exposure on glutathione homeostasis in human bronchial epithelial cells. Cell Physiol Biochem. 2013;32(7):129–45.
 
25.
Church DF, Pryor WA. Free-radical chemistry of cigarette smoke and its toxicological implications. Environ Health Perspect. 1985;64:111–26.
 
26.
Scian MJ, Oldham MJ, Kane DB, Edmiston JS, McKinney WJ. Characterization of a whole smoke in vitro exposure system (Burghart Mimic Smoker-01). Inhal Toxicol. 2009;21(3):234–43.
 
27.
Ahmed A, Thliveris JA, Shaw A, Sowa M, Gilchrist J, Scott JE. Caspase 3 activity in isolated fetal rat lung fibroblasts and rat periodontal ligament fibroblasts: cigarette smoke induced alterations. Tob Induc Dis. 2013;11(1):25.
 
28.
Kimball JR, Nittayananta W, Klausner M, Chung WO, Dale BA. Antimicrobial barrier of an in vitro oral epithelial model. Arch Oral Biol. 2006;51(9):775–83.
 
29.
Shibata Y, Abe S, Inoue S, Takabatake N, Igarashi A, Takeishi Y, et al. Altered expression of antimicrobial molecules in cigarette smoke-exposed emphysematous mice lungs. Respirology. 2008;13(7):1061–5.
 
30.
Prado-Montes de Oca E. Human beta-defensin 1: a restless warrior against allergies, infections and cancer. Int J Biochem Cell Biol. 2010;42(6):800–4.
 
31.
Chen L, Sun BB, Wang T, Wang X, Li JQ, Wang HX, et al. Cigarette smoke enhances {beta}-defensin 2 expression in rat airways via nuclear factor-{kappa}B activation. Eur Respir J. 2010;36(3):638–45.
 
32.
De Smet K, Contreras R. Human antimicrobial peptides: defensins, cathelicidins and histatins. Biotechnol Lett. 2005;27(18):1337–47.
 
33.
Winter J, Pantelis A, Reich R, Martini M, Kraus D, Jepsen S, et al. Human beta-defensin-1, -2, and -3 exhibit opposite effects on oral squamous cell carcinoma cell proliferation. Cancer Invest. 2011;29(3):196–201.
 
34.
Nikota JK, Stampfli MR. Cigarette smoke-induced inflammation and respiratory host defense: insights from animal models. Pulm Pharmacol Ther. 2012;25(4):257–62.
 
35.
Mahanonda R, Sa-Ard-Iam N, Eksomtramate M, Rerkyen P, Phairat B, Schaecher KE, et al. Cigarette smoke extract modulates human beta-defensin-2 and interleukin-8 expression in human gingival epithelial cells. J Periodontal Res. 2009;44(4):557–64.
 
36.
Schauber J, Dorschner RA, Yamasaki K, Brouha B, Gallo RL. Control of the innate epithelial antimicrobial response is cell-type specific and dependent on relevant microenvironmental stimuli. Immunology. 2006;118(4):509–19.
 
37.
Carpenter S, Ricci EP, Mercier BC, Moore MJ, Fitzgerald KA. Post-transcriptional regulation of gene expression in innate immunity. Nat Rev Immunol. 2014;14(6):361–76.
 
38.
Mulder KC, Bandola J, Schumann W. Construction of an artificial secYEG operon allowing high level secretion of alpha-amylase. Protein Expr Purif. 2013;89(1):92–6.
 
 
CITATIONS (15):
1.
Benefits of Bifidobacterium animalis subsp. lactis Probiotic in Experimental Periodontitis
Luiz F.F. Oliveira, Sérgio L. Salvador, Pedro H.F. Silva, Flávia A.C. Furlaneto, Luciene Figueiredo, Renato Casarin, Edilson Ervolino, Daniela B. Palioto, Sérgio L.S. Souza, Mario Taba, Arthur B. Novaes, Michel R. Messora
Journal of Periodontology
 
2.
Antimicrobial and antiviral effects of human defensins: pathogenetic value and prospective application to medicinal therapy
Vladimir I Vaschenko, Vladimir N Vil’yaninov, Petr D Shabanov
Reviews on Clinical Pharmacology and Drug Therapy
 
3.
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Sheng Ge, Pei Ye, Guo-Yang Li, Yi-Fu Fu, Qian Zhou, Fan Huang, Xiang Wang, Wen-Mei Wang
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7.
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Pei Ye, Xiang Wang, Sheng Ge, Wei Chen, Wenmei Wang, Xiaodong Han
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8.
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Ying Gao, Guanzhao Liang, Qiong Wang, Xiaodong She, Dongmei Shi, Yongnian Shen, Xiaohong Su, Xiang Wang, Wenmei Wang, Dongmei Li, Weida Liu
Mycopathologia
 
9.
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Qin Liu, Maomao Zhao, Wei Chen, Kaiyuan Xu, Fan Huang, Junxing Qu, Zhen Xu, Xiang Wang, Yong Wang, Yanan Zhu, Wenmei Wang
Archives of Oral Biology
 
10.
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Na An, Jasmin Holl, Xuekui Wang, Marco Rausch, Oleh Andrukhov, Xiaohui Rausch-Fan
International Journal of Environmental Research and Public Health
 
11.
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Frontiers in Physiology
 
12.
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Mikhlid Almutairi, Bader Almutairi, Mohammad Almutairi, Narasimha Parine, Abdulwahed Alrefaei, Mohammad Alanazi, Abdelhabib Semlali
Environmental Science and Pollution Research
 
13.
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Potravinarstvo Slovak Journal of Food Sciences
 
14.
Antimicrobial Peptides and Interleukins in Cleft Soft Palate
Arina Deņisova, Māra Pilmane, Dzintra Kažoka
Children
 
15.
Attenuation of NLRP3 Inflammasome by Cigarette Smoke is Correlated with Decreased Defense Response of Oral Epithelial Cells to Candida albicans
Fan Huang, Ruiqi Xie, Ruowei Li, Liu Liu, Maomao Zhao, Qiong Wang, Weida Liu, Pei Ye, Wenmei Wang, Xiang Wang
Current Molecular Medicine
 
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