RESEARCH PAPER
Tobacco Xenobiotics Release Nitric Oxide
| 1 | Department of Dentistry, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Canada |
| 2 | Free Radical & Radiation Biology Graduate Program and Electron Spin Resonance Facility,
The University of Iowa College of Medicine, Iowa City, Iowa, USA |
CORRESPONDING AUTHOR
EWN Lam
Faculty of Medicine and Dentistry, University of Alberta, DPC 2085, Edmonton, AB T6G 2N8, Canada
Faculty of Medicine and Dentistry, University of Alberta, DPC 2085, Edmonton, AB T6G 2N8, Canada
Publish date: 2009-09-15
Tobacco Induced Diseases 2003;1(September):207
KEYWORDS
nicotineelectron paramagnetic resonanceelectron paramagnetic resonance spectrumelectron paramagnetic resonance signalsmokeless tobacco
ABSTRACT
Many xenobiotic compounds exert their actions through the release of free
radicals and related oxidants 1,2, bringing about unwanted biological effects 3. Indeed,
oxidative events may play a significant role in tobacco toxicity from cigarette smoke. Here,
we demonstrate the direct in vitro release of the free radical nitric oxide (•
NO) from extracts
and components of smokeless tobacco, including nicotine, nitrosonornicotine (NNN) and 4-
(methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone (NNK) in phosphate buffered saline and
human saliva using electron spin resonance and chemiluminescence detection. Our findings
suggest that tobacco xenobiotics represent as yet unrecognized sources of •
NO in the body.
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