Tobacco Xenobiotics Release Nitric Oxide
EWN Lam 1  
EE Kelley 2
SM Martin 2
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Department of Dentistry, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Canada
Free Radical & Radiation Biology Graduate Program and Electron Spin Resonance Facility, The University of Iowa College of Medicine, Iowa City, Iowa, USA
EWN Lam   

Faculty of Medicine and Dentistry, University of Alberta, DPC 2085, Edmonton, AB T6G 2N8, Canada
Publish date: 2009-09-15
Tobacco Induced Diseases 2003;1(September):207
Many xenobiotic compounds exert their actions through the release of free radicals and related oxidants 1,2, bringing about unwanted biological effects 3. Indeed, oxidative events may play a significant role in tobacco toxicity from cigarette smoke. Here, we demonstrate the direct in vitro release of the free radical nitric oxide (• NO) from extracts and components of smokeless tobacco, including nicotine, nitrosonornicotine (NNN) and 4- (methyl-N-nitrosamino)-1-(3-pyridyl)-1-butanone (NNK) in phosphate buffered saline and human saliva using electron spin resonance and chemiluminescence detection. Our findings suggest that tobacco xenobiotics represent as yet unrecognized sources of • NO in the body.
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