Mitogenic and functional responses by nicotine and hydrogen peroxide in AR42J cells: a comparative study
More details
Hide details
Department of Physiology and Biophysics, University of Arkansas for Medical Sciences, Little Rock, USA
Department of Geriatrics, University of Arkansas for Medical Sciences, Little Rock, USA
Medical Research, Central Arkansas Veterans Healthcare System, Little Rock, USA
Submission date: 2008-06-13
Acceptance date: 2008-07-31
Publication date: 2008-07-31
Tobacco Induced Diseases 2008;4(July):5
The aim of the current study was to investigate the oxidative effects of nicotine by examining the mitogenic and functional responses in AR42J cells. As a control and for comparison, hydrogen peroxide (H2O2) was used as a source of known oxidative biomarker. Responses were examined by determining cell proliferation through the activation of ERK signaling, basal and CCK-stimulated cell function and measuring lipid peroxidation. AR42J cells have been exposed to either a non-cytotoxic dose of 20 μM H2O2 for 15 min or to 100 μM of nicotine for 3 min respectively. Nicotine and H2O2 at these dose and time intervals produced similar levels of malondialdyde (MDA) production and p-ERK1/2 activation. Immunofluorescence studies employing specific antibody to p-ERK1/2 confirmed the latter. Nicotine-induced increase in the proliferation of AR42J cells was significantly higher in comparison to H2O2 exposed cells. CCK-stimulated cell function induced by nicotine was significantly higher in AR42J cells as compared to the response by H2O2. These results suggest that nicotine- induced mitogenic and functional response in AR42J cells are associated with ERK signaling and increase in reactive oxygen species production. The data suggests that nicotine-induced mitogenic response in AR42J cells closely identifies the response induced by an oxidative biomarker.
Mokdad AH, Marks JS, Stroup DF, Gerberding JL: Actual causes of death in the United States, 2000. JAMA. 2004, 291: 1238-1245. 10.1001/jama.291.10.1238.
Morbidity and Mortality Weekly Report, Tobacco use–United States. 1900–1999. MMWR Morb Mortal Wkly Rep. 1999, 48: 986-993.
Talamini G, Bassi C, Falconi M, Sartori N, Salvia R, Rigo L, Castagnini A, Di FV, Frulloni L, Bovo P, Vaona B, Angelini G, Vantini I, Cavallini G, Pederzoli P: Alcohol and smoking as risk factors in chronic pancreatitis and pancreatic cancer. Dig Dis Sci. 1999, 44: 1303-1311. 10.1023/A:1026670911955.
Lin Y, Tamakoshi A, Hayakawa T, Ogawa M, Ohno Y: Cigarette smoking as a risk factor for chronic pancreatitis: a case-control study in Japan. Research Committee on Intractable Pancreatic Diseases. Pancreas. 2000, 21: 109-114. 10.1097/00006676-200008000-00001.
Rayford PL, Chowdhury P: Mecamylamine, a nicotinic receptor channel antagonist, affects amylase secretion by isolated pancreatic acinar cells. J Assoc Acad Minor Phys. 2001, 12: 105-108.
Chowdhury P, MacLeod S, Udupa KB, Rayford PL: Pathophysiological effects of nicotine on the pancreas: an update. Exp Biol Med (Maywood). 2002, 227: 445-454.
Wetscher GJ, Bagchi M, Bagchi D, Perdikis G, Hinder PR, Glaser K, Hinder RA: Free radical production in nicotine treated pancreatic tissue. Free Radic Biol Med. 1995, 18: 877-882. 10.1016/0891-5849(94)00221-5.
Yildiz D, Liu YS, Ercal N, Armstrong DW: Comparison of pure nicotine- and smokeless tobacco extract-induced toxicities and oxidative stress. Arch Environ Contam Toxicol. 1999, 37: 434-439. 10.1007/s002449900537.
Park BK, Chung JB, Lee JH, Suh JH, Park SW, Song SY, Kim H, Kim KH, Kang JK: Role of oxygen free radicals in patients with acute pancreatitis. World J Gastroenterol. 2003, 9: 2266-2269.
Bose C, Zhang H, Udupa KB, Chowdhury P: Activation of p-ERK1/2 by nicotine in pancreatic tumor cell line AR42J: effects on proliferation and secretion. Am J Physiol Gastrointest Liver Physiol. 2005, 289: G926-G934. 10.1152/ajpgi.00138.2005.
Chowdhury P, Bose C, Udupa K: Nicotine induced proliferation of isolated rat pancreatic acinar cells: Effect of cell signaling and function. Cell Proliferation. 2007, 40: 125-141. 10.1111/j.1365-2184.2007.00418.x.
Chiarugi P: Reactive oxygen species as mediators of cell adhesion. Ital J Biochem. 2003, 52: 28-32.
Ganesh PC, Rao SMN: Evidence for oxidant stress in chronic pancreatitis. Indian J Gastroenterol. 1999, 18: 156-157.
Piperakis SM, Visvardis EE, Sagnou M, Tassiou AM: Effects of smoking and aging on oxidative DNA damage of human lymphocytes. Carcinogenesis. 1998, 19: 695-698. 10.1093/carcin/19.4.695.
Christophe J: Pancreatic tumoral cell line AR42J: an amphicrine model. Am J Physiol. 1994, 266: G963-G971.
Soulsby ME, Phillips B, Chowdhury P: The effects of a soy-protein diet o elevated brain lipid peroxide levels induced by simulated weightlessness. Annals of Clin & Lab Sci. 2004, 34: 103-106.
Chowdhury P, Soulsby M, Kim K: L-carnitine influence on oxidative stress induced by hind-limb unloading in Adult Rats. Aviat Space Environ Med. 2007, 78: 554-556.
Bradford MM: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976, 72: 248-254. 10.1016/0003-2697(76)90527-3.
Jung DH: Preparation and application of Procion Yellow starch for amylase assay. Clin Chim Acta. 1980, 100: 7-11. 10.1016/0009-8981(80)90179-5.
Zhou Y, Wang Q, Evers BM, Chung DH: Signal transduction pathways involved in oxidative stress-induced intestinal epithelial cell apoptosis. Pediatr Res. 2005, 58: 1192-1197. 10.1203/01.pdr.0000185133.65966.4e.
Watanabe N, Zmijewski JW, Takabe W, Umezu-Goto M, Le Goffe C, Sekine A, Landar A, Watanabe A, Aoki J, Arai H, Kodama T, Murphy MP, Kalyanaraman R, Darley-Usmar VM, Noguchi N: Activation of mitogen-activated protein kinases by lysophosphatidylcholine-induced mitochondrial reactive oxygen species generation in endothelial cells. Am J Pathol. 2006, 168: 737-1748. 10.2353/ajpath.2006.050648.
Chowdhury P, Hosotani R, Rayford PL: Inhibition of CCK or carbachol stimulated amylase release by nicotine. Life Sciences. 1989, 45: 2163-2168. 10.1016/0024-3205(89)90083-0.
Ogawa Y, Kobayashi T, Nishioka A, Kariya S, Ohnishi T, Hamasato S, Seguchi H, Yoshida S: Reactive oxygen species-producing site in radiation-induced apoptosis of human peripheral T cells: involvement of lysosomal membrane destabilization. Int J Mol Med. 2004, 13: 69-73.
Riley PA: Free radicals in biology: oxidative stress and the effects of ionizing radiation. Int J Radiat Biol. 1994, 65: 27-33. 10.1080/09553009414550041.
Iijima R, Takahashi H, Namme R, Ikegami S, Yamazaki M: Novel biological function of sialic acid (N-acetylneuraminic acid) as a hydrogen peroxide scavenger. FEBS Lett. 2004, 561: 163-166. 10.1016/S0014-5793(04)00164-4.
Baud O, Greene AE, Li J, Wang H, Volpe JJ, Rosenberg PA: Glutathione peroxidase-catalase cooperativity is required for resistance to hydrogen peroxide by mature rat oligodendrocytes. J Neurosci. 2004, 24: 1531-1540. 10.1523/JNEUROSCI.3989-03.2004.
Meiners S, Ludwig A, Lorenz M, Dreger H, Baumann G, Stangl V, Stangl K: Nontoxic proteasome inhibition activates a protective antioxidant defense response in endothelial cells. Free Radic Biol Med. 2006, 40: 2232-2241. 10.1016/j.freeradbiomed.2006.03.003.
Martindale JL, Holbrook NJ: Cellular response to oxidative stress: signaling for suicide and survival. J Cell Physiol. 2002, 192: 1-15. 10.1002/jcp.10119.
Yang B, Oo TN, Rizzo V: Lipid rafts mediate H2O2 prosurvival effects in cultured endothelial cells. FASEB J. 2006, 20: 1501-1503. 10.1096/fj.05-5359fje.
Cobb MH: MAP kinase pathways. Prog Biophys Mol Biol. 1999, 71: 479-500. 10.1016/S0079-6107(98)00056-X.
Zhang J, Jin N, Liu Y, Rhoades RA: Hydrogen peroxide stimulates extracellular signal-regulated protein kinases in pulmonary arterial smooth muscle cells. Am J Respir Cell Mol Biol. 1998, 19: 324-332.
Song HJ, Lee TS, Jeong JH, Min YS, Shin C, Sohn UD: Hydrogen peroxide-induced extracellular signal-regulated kinase activation in cultured feline ileal smooth muscle cells. J Pharmacol Exp Ther. 2005, 312: 391-398. 10.1124/jpet.104.074401.
Granados MP, Salido GM, Pariente JA, Gonzales A: Effect of H2O2 on CCK-8-evoked changes in mitochondial activity in isolated mouse pancreatic acinar cells. Biology of the Cell. 2005, 97: 847-856. 10.1042/BC20040513.
Weitberg AB, Corvese D: Oxygen radicals potentiate the genetic toxicity of tobacco-specific nitrosamines. Clin Genet. 1993, 43: 88-91.
Wittel UA, Pandey KK, Andranifahanana M, Johansson SL, Cullen DM, Akhter MP, Brand RE, Prokopczyk B, Batra SK: Chronic pancreatic inflammation induced by environmental tobacco smoke inhalation in rats. Am J Gastroenterol. 2006, 101: 148-159. 10.1111/j.1572-0241.2006.00405.x.
Menthol-enhanced cytotoxicity of cigarette smoke demonstrated in two bioassay models
Atsuko Noriyasu, Tadashi Konishi, Shinichi Mochizuki, Kazuo Sakurai, Yutaka Tanaike, Ken Matsuyama, Kazuya Uezu, Tomonori Kawano
Tobacco Induced Diseases
Mechanisms for redox actions of nicotine and glutathione in cell culture, relevant to periodontitis
Federico Tinti, Mena Soory
Scientific Reports
Anabolic Actions of the Regenerative Agent Enamel Matrix Derivative (EMD) in Oral Periosteal Fibroblasts and MG 63 Osteoblasts, Modulation by Nicotine and Glutathione in a Redox Environment
Tareq Al-Qattan, Mena Soory
Journal of Functional Biomaterials
Lethal impacts of cigarette smoke in cultured tobacco cells
Masaru Yukihiro, Takuya Hiramatsu, Tomonori Kawano
Tobacco Induced Diseases