RESEARCH PAPER
Effects of whole cigarette smoke on human beta defensins expression and secretion by oral mucosal epithelial cells
Wen-mei Wang 1  
,  
Pei Ye 1
,  
Ya-jie Qian 1
,  
Ya-fan Gao 1
,  
Jing-jing Li 1
,  
Fang-fang Sun 2, 3
,  
Wei-yun Zhang 3
,  
Xiang Wang 1, 3  
 
 
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1
Department of Oral Medicine, Institute and Hospital of Stomatology, Nanjing University Medical School, Nanjing, China
2
Department of Prosthodontics, Institute and Hospital of Stomatology, Nanjing University Medical School, Nanjing, China
3
Immunology and Reproduction Biology Laboratory, Medical School, Nanjing University, Nanjing, China
CORRESPONDING AUTHOR
Wen-mei Wang   

Department of Oral Medicine, Institute and Hospital of Stomatology, Nanjing University Medical School, 30 Zhongyang Road, Nanjing 210008, China
Xiang Wang   

Immunology and Reproduction Biology Laboratory, Medical School, Nanjing University, 22 Hankou Road, Nanjing 210093, China
Publish date: 2015-01-24
 
Tobacco Induced Diseases 2015;13(January):3
KEYWORDS
ABSTRACT
Background:
Cigarette smoke a recognized risk factor for many systemic diseases and also oral diseases. Human beta defensins (HBDs), a group of important antimicrobial peptides expressed by the epithelium, are crucial for local defense and tissue homeostasis of oral cavity. The aim of this study was to evaluate potential effects of whole cigarette smoke (WCS) exposure on the expression and secretion of HBDs by oral mucosal epithelial cells.

Methods:
Immortalized human oral mucosal epithelial (Leuk-1) cells were exposed to WCS for various time periods. HBD-1, -2 and -3 expression and subcellular localization were detected by real time qPCR, immunofluorescence assay and confocal microscopy. According to the relative fluorescent intensity, the expression levels of HBD-1, -2 and -3 were evaluated by digital image analysis system. The alteration of HBD-1, -2 and -3 secretion levels was measured by the Enzyme-Linked Immunosorbent Assay.

Results:
WCS exposure remarkably attenuated HBD-1 expression and secretion while clearly enhanced HBD-2, -3 expression levels and HBD-2 secretion by Leuk-l cells. It appeared that there was no significant effect of WCS exposure on HBD-3 secretion.

Conclusions:
WCS exposure could modulate expression and secretion of HBDs by oral mucosal epithelial cells, establishing a link between cigarette smoke and abnormal levels of antimicrobial peptides. The present results may give a new perspective to investigate smoking-related local defense suppression and oral disease occurrence.

 
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