RESEARCH PAPER
Combined effects of cigarette smoking, alcohol drinking and eNOS Glu298Asp polymorphism on blood pressure in Chinese male hypertensive subjects
Zhe Hong 1
,  
Liying Pan 2
,  
Zhangqing Ma 3
,  
Yue Zhu 3
,  
 
 
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1
Tongji Hospital of Tongji University School of Medicine, Shanghai, China
2
The First Affiliated Hospital of Wannan Medical College, Wuhu, China
3
Laboratory of Quantitative Pharmacology, Wannan Medical College, Wuhu, China
CORRESPONDING AUTHOR
Zongyuan Hong   

Laboratory of Quantitative Pharmacology, Wannan Medical College, No. 22, Wenchang West Road, Wuhu 241002, Anhui, China
Publish date: 2019-08-02
 
Tob. Induc. Dis. 2019;17(August):59
KEYWORDS
TOPICS
ABSTRACT
Introduction:
Genetic factors and lifestyle exposures, as well as their combinations, play important roles in the development of hypertension. We examined whether cigarette smoking, alcohol drinking and the Glu298Asp polymorphism of the endothelial nitric oxide synthase (eNOS) gene generate combined effects on blood pressure (BP) in hypertensive subjects.

Methods:
A total of 342 essential hypertensive subjects were recruited from Susong community in Anhui province, China, from July 2017 to January 2018, and the plasma biochemical parameters and the genotype on Glu298Asp polymorphism were determined.

Results:
There were no gender differences in the distributions of alleles and genotypes in hypertensive subjects. The proportions of cigarette smoking and alcohol drinking in male hypertensive subjects were remarkably higher than those in the females (p<0.001). The systolic blood pressure (SBP) and diastolic blood pressure (DBP) levels of mutant genotypes (Glu/Asp and Asp/Asp) were significantly higher than those of wild genotype (Glu/Glu) (p=0.013 and 0.026, respectively) in male hypertensive subjects. Moreover, the SBP and DBP levels of the mutant genotype were remarkably higher than those of wild genotype in both cigarette smoking and alcohol drinking male hypertensive subjects (p=0.034 and 0.043, respectively).

Conclusions:
Cigarette smoking, alcohol drinking and the Glu298Asp polymorphism of the eNOS gene generate combined effects that increase the susceptibility of the mutant genotype to BP in Chinese male hypertensive subjects.

ABBREVIATIONS
ANCOVA: analysis of covariance, BMI: body mass index, CI: confidence interval, CHOL: total cholesterol, DBP: diastolic blood pressure, eNOS: endothelial nitric oxide synthase, EH: essential hypertension, HDL: high-density lipoprotein, LDL: low-density lipoprotein, NO: nitric oxide, OR: odds ratio, SD: standard deviation, SE: standard error, SBP: systolic blood pressure, TG: total triglyceride
ACKNOWLEDGEMENTS
We are indebted to the patients who participated in the study and to their families.
CONFLICTS OF INTEREST
The authors have completed and submitted the ICMJE Form for Disclosure of Potential Conflicts of Interest and none was reported.
FUNDING
This study was supported in part by grants-in-aid from the National Natural Science Foundation of China (proposal number 81671318 and 81171255), the Key Project of Chinese Ministry of Education (proposal number 208058) and the Programs for Science and Technology Development of Anhui Province (proposal number 1501041157).
AUTHORS' CONTRIBUTIONS
Z.H., Z.M., Y.Z. and L.P. acquired the data and performed statistical analyses. Z.H. drafted the manuscript. Z.H. and Z.H. conceived the study, participated in its design and coordination, and revised the manuscript for important intellectual content. All authors contributed to the study design and data interpretation, and read and approved the final manuscript.
PROVENANCE AND PEER REVIEW
Not commissioned; externally peer reviewed.
 
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