RESEARCH PAPER
Chemical constituents of tobacco smoke induce the production of interleukin-8 in human bronchial epithelium, 16HBE cells
Guojun Zhou 1  
,  
Weiqiang Xiao 1
,  
Chengyun Xu 2
,  
Yajun Hu 1
,  
Xiaokai Wu 2
,  
Xinbo Lu 1
,  
Chunyun Shi 1
,  
Ximei Wu 2  
 
 
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1
Technological Center of China Tobacco Zhejiang Industrial Co., LTD., Hangzhou, China
2
Department of Pharmacology, School of Medicine, Zhejiang University, Hangzhou, China
CORRESPONDING AUTHOR
Guojun Zhou   

Technological Center of China Tobacco Zhejiang Industrial Co., LTD., 118 Kehai Road, Hangzhou, Zhejiang Province 310024, China
Ximei Wu   

Department of Pharmacology, School of Medicine, Zhejiang University, 866 Yuhangtang Road, Hangzhou 310058, China
Publish date: 2016-07-19
 
Tobacco Induced Diseases 2016;14(July):24
KEYWORDS
ABSTRACT
Background:
Interleukin-8 (IL-8) functions as a major chemoattractant and plays pivotal roles in the initiation and development of chronic obstructive pulmonary disease (COPD), and tobacco smoke is a most risk factor contributing to the development of COPD. Hence, we have screened some of the tobacco smoke-derived chemical compounds that potentially induce the production of IL-8 in human bronchial epithelium, 16HBE cells.

Methods:
Interleukin-8 (IL-8) functions as a major chemoattractant and plays pivotal roles in the initiation and development of chronic obstructive pulmonary disease (COPD), and tobacco smoke is a most risk factor contributing to the development of COPD. Hence, we have screened some of the tobacco smoke-derived chemical compounds that potentially induce the production of IL-8 in human bronchial epithelium, 16HBE cells.

Results:
At the non-toxic dosages, chemical compounds belonging to nicotine, aromatic amines, benzopyrene, phenols, aldehydes, and some other volatile organics dose-dependently increased IL-8 reporter gene expression. Consistently, the representative compounds belonging to nicotine, aromatic amines, benzopyrene, phenols, aldehydes, and some other volatile organics significantly and dose-dependently increased IL-8 levels in the culture supernatants of 16HBE cells, among these compounds, benzopyrene is a most potent stimulator for inducing IL-8 production.

Conclusions:
The present study has identified particular tobacco smoke constituents responsible for inducing the IL-8 production in human bronchial epithelium, which might help shed light on the pathogenesis of tobacco smoke-induced COPD.

 
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